The Core is experienced in post-hybridoma antibody development and validation procedures that include isotyping, determination of antibody specificity, determination of CDR sequences of variable H and L chains and determination of antibody affinity by Biacore. Our core offers highly competitive prices for several antibody characterization services and significant discounts for multiple antibodies characterized and analyzed in parallel.
We offer extremely fast isotype testing service for hybridoma culture supernatants and purified antibodies. We also perform custom antibody specificity determination assays such as ELISA (both Indirect and Capture), western blot and FACS. The MAC also offers antibody validation services for purified antibodies, hybridoma culture supernatants and serum samples from immunized animals as well as custom biomarker validation and assay development services. We make every effort to validate antibodies by testing various conditions for biomarker studies.