\ Antibody Generation (mAb/pAb) | Monoclonal Antibody Core

Antibody Generation (mAb/pAb)

The process for making a monoclonal antibody starts with immunizing an animal with a target antigen, generating a sufficient immune response to the immunogen, and then fusing the B cell making the antibody with a myeloma fusion partner to create a hybridoma. Success depends on matching the best immunogen, the right animal species/strain, and then screening the fusion plates using the correct assay to identify the antibodies with the required reactivity characteristics.
 

Monoclonal antibodies

Immunizations (mouse, rat, hamster)

 

Polyclonal antibodies

Immunizations (rabbit)