\ FAQ / Links | Monoclonal Antibody Core

FAQ / Links

 

Frequently Asked Questions:

 

I am interested in generating monoclonal antibodies in the MAC. Where do I start?

There are a wide variety of approaches available; therefore researchers are encouraged to make a consultation appointment with the Core Director at 617-632-2209. For the consultation appointment, please have the sequence of your protein available, the species from which is it derived and a good idea of how you will use the antibodies in your experiments i.e. Western blot analysis, IHC, IF, flow cytometry, or ELISA detection.
 

What species will be used to generate my mAbs?

The host selected for your immunization will be dependent on the type of protein (intracellular vs. extracellular) and the species from which the protein is derived.

 

I want my mAbs to function in a specific assay that I’ve designed. May I screen for these antibodies myself?

Yes. You need to tell us at the consultation that you will be screening the antibodies. It will be very important to coordinate the timing of the screening to prevent overgrowth of the cells.

 

How can I find out about progress of my project?

The generation of mAbs is a long and multi-step procedure. The MAC will contact you by phone or send e-mail notification once a distinct step is completed and requires your permission to proceed to the next one (for example, once the animal shows an immune response in the sera screen and is ready for fusion). Information on any project can be obtained at any time by contacting the MAC Director or scientist assigned to your project.

 

How do I get billed?

We request information about your financial coordinator (on the account initiation form), billing address, etc. and if you or your PI works at the DFCI, we can directly bill your cost center.  For outside researchers, we also require a PO (hard copy) and the appropriate billing information.

 

Do you guarantee that I will get an antibody for my project?

Every antibody development project has its risks; one of them is that we cannot guarantee that your protein will be immunogenic. We have quality control measures in place that will help increase the chances of success, software programs to analyze antigens and a very good design team but there are always proteins that fail to produce an antibody despite every prediction. Our director has been generating antibodies for over 30 years, bringing a vast amount of experience to bear, providing every project its best chance for success.

 


 

Useful Links:

Antibody Resource Page: The Antibody Resource Page is the foremost internet
resource on antibodies providing extensive information about antibodies; what
they are, how they work, who sells them, etc. Their website and its content
have been designed and contributed by a mixture of scientists and antibody
industry veterans, giving it their perspective from both customer and supplier to
merge their interests.
http://www.antibodyresource.com/

Antibodypedia: The Antibodypedia is an open-access database of publicly
available antibodies against human protein targets. The site features user and
provider data on antibody efficacy in a range of biochemical and cell biological
techniques--so you can find the right antibody for the right application.
http://www.antibodypedia.com/

IHC World: IHC World is a life science company with broad range of products
and services. We provide information, advertising, marketing and sales,
products and services in the field of histology, histopathology,
immunohistochemistry, electron microscopy, tissue microarray, biospecimen
including cell and tissue bank. This includes protocols and a knowledge center.
http://www.ihcworld.com/

CSH Press: Cold Spring Harbor Laboratory Press is an internationally
renowned publisher of books, journals, and electronic media, located on
Long Island, New York. Since 1933, it has furthered the advance and
spread of scientific knowledge in all areas of genetics and molecular
biology, including cancer biology, plant science, bioinformatics, and
neurobiology. It is a division of Cold Spring Harbor Laboratory, an
innovator in life science research and the education of scientists,
students, and the public.
http://www.cshlpress.com/

21st Century Biochemicals, Inc.: 21st Century Biochemicals offer the
highest quality Epigenetic Peptides, Custom Peptides, Custom
Antibodies, Antibodies to Selected Targets and Bioactive Peptides.
They are very knowledgeable and professional.
http://www.21stcenturybio.com/

ATCC: ATCC is an independent, private, nonprofit biological resource
center that authenticates microorganisms and cell lines and manages
logistics of long-term preservation and distribution of cultures for the
scientific community. It is also a research organization working to
generate new knowledge new in vitro model systems, describing new
species, search for new disease biomarkers and build bodies of
characterization data for valuable biological materials.
http://www.atcc.org/

ExPASy: ExPASy is the SIB Bioinformatics Resource Portal which provides
access to scientific databases and software tools in different areas of life
sciences including proteomics, genomics, phylogeny, systems biology,
population genetics, transcriptomics etc. On this portal you find resources
from many different SIB groups as well as external institutions.
http://www.expasy.org/

Immune Epitope Database: The IEDB contains data related to antibody
and T cell epitopes for humans, non-human primates, rodents, and other
animal species. Curation of peptidic and non-peptidic epitope data relating
to all infectious diseases, allergens, autoimmune diseases, and
transplant/alloantigens is current and constantly being updated. The
ase also contains MHC binding data from a variety of different
antigenic sources and immune epitope data from the FIMM (Brusic), HLA
Ligand (Hildebrand), TopBank (Sette), and MHC binding (Buus)
databases.
http://www.iedb.org/

The Developmental Studies Hybridoma Bank: The DSHB was created
to bank and distribute hybridomas and the monoclonal antibodies (MAbs)
to the general scientific community in order to facilitate research.
http://dshb.biology.uiowa.edu/